{"id":5159,"date":"2019-03-10T20:30:37","date_gmt":"2019-03-10T19:30:37","guid":{"rendered":"http:\/\/bifi.es\/schedule\/bifi-talk-javier-garcia-nafria\/"},"modified":"2019-09-17T09:09:14","modified_gmt":"2019-09-17T07:09:14","slug":"bifi-talk-javier-garcia-nafria","status":"publish","type":"tribe_events","link":"https:\/\/bifi.es\/es\/schedule\/bifi-talk-javier-garcia-nafria\/","title":{"rendered":"BIFI TALK – Javier Garc\u00eda-Nafr\u00eda"},"content":{"rendered":"

T\u00edtulo: Cryo-electron microscopy: moving beyond X-ray crystallography for membrane receptors and drug discovery
\n<\/strong><\/p>\n

Ponente: <\/strong>Javier Garc\u00eda-Nafr\u00eda (LMB\/MRC, Cambridge, UK-University of Zaragoza)<\/p>\n

D\u00eda y hora:\u00a0 <\/strong>4 de octubre\u00a0a las 13:00 h<\/p>\n

Lugar:\u00a0<\/strong>SALA DE CONFERENCIAS, EDIFICIO I+D+i, CAMPUS RIO EBRO<\/p>\n

Resumen:<\/strong><\/p>\n

Cryo-electron microscopy (cryo-EM) has undergone major advances during the last years, with special impact in structural biology of membrane proteins. I will review the advances in cryo-EM technology that made the \u00a8resolution revolution\u00a8 possible, and then show its application to understand G-protein coupled receptors (GPCRs). GPCRs are the largest family of membrane proteins in the human body and are a target for ~35% of the currently approved FDA drugs. GPCRs sense a variety of molecules and transduce the signal to the intracellular milieu by coupling to heterotrimeric G-proteins. We used cryo-EM to obtain high-resolution structural information on GPCR-G protein complexes, starting to tackle one of the major questions in the field \u2013 the GPCR-G protein selectivity code. I will then analyse the state-of-the-art in using cryo-EM for structure-based drug discovery of membrane proteins.<\/p>\n

References:<\/p>\n

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  1. Cryo-EM structure of the adenosine A2A<\/sub> receptor coupled to an engineered heterotrimeric G protein. <\/em>Garc\u00eda-Nafr\u00eda J.*, Lee Y.*, Bai X., Carpenter B. & Tate CG. 2018. Elife. May 4;7. pii: e35946.<\/li>\n<\/ol>\n
      \n
    1. Cryo-EM structure of the serotonin 5-HT1B receptor coupled to heterotrimeric Go. <\/em>Garc\u00eda-Nafr\u00eda J., Nehme R., Edwards P., Tate CG. 2018. Nature 558 (7711). 620-62<\/li>\n<\/ol>\n
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      1. Cryo-EM structures of GPCRs coupled to Gs, Gi and Go<\/em>.\u00a0 Garc\u00eda-Nafr\u00eda J. and Tate CG. Molecular and Cellular Endocrinology.<\/em> 2019, In press.<\/li>\n<\/ol>\n
          \n
        1. Cryo-Electron Microscopy: Moving Beyond X-ray Crystal Structures for Drug receptors and Drug development. <\/em>Garc\u00eda-Nafr\u00eda J. and Tate CG. 2019. Annual Reviews in Pharmacology and Toxicology. 2020. In press (2019 Jul 26. doi: 10.1146.)
          \n<\/strong><\/li>\n<\/ol>\n

           <\/p>\n","protected":false},"excerpt":{"rendered":"

          T\u00edtulo: Cryo-electron microscopy: moving beyond X-ray crystallography for membrane receptors and drug discovery Ponente: Javier Garc\u00eda-Nafr\u00eda (LMB\/MRC, Cambridge, UK-University of Zaragoza) D\u00eda y hora:\u00a0 4 de octubre\u00a0a las 13:00 h […]<\/p>\n","protected":false},"author":8,"featured_media":0,"template":"","meta":{"_tribe_events_status":"","_tribe_events_status_reason":"","footnotes":""},"tags":[],"tribe_events_cat":[33],"class_list":["post-5159","tribe_events","type-tribe_events","status-publish","hentry","tribe_events_cat-eventos","cat_eventos"],"_links":{"self":[{"href":"https:\/\/bifi.es\/es\/wp-json\/wp\/v2\/tribe_events\/5159","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bifi.es\/es\/wp-json\/wp\/v2\/tribe_events"}],"about":[{"href":"https:\/\/bifi.es\/es\/wp-json\/wp\/v2\/types\/tribe_events"}],"author":[{"embeddable":true,"href":"https:\/\/bifi.es\/es\/wp-json\/wp\/v2\/users\/8"}],"version-history":[{"count":1,"href":"https:\/\/bifi.es\/es\/wp-json\/wp\/v2\/tribe_events\/5159\/revisions"}],"predecessor-version":[{"id":5317,"href":"https:\/\/bifi.es\/es\/wp-json\/wp\/v2\/tribe_events\/5159\/revisions\/5317"}],"wp:attachment":[{"href":"https:\/\/bifi.es\/es\/wp-json\/wp\/v2\/media?parent=5159"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bifi.es\/es\/wp-json\/wp\/v2\/tags?post=5159"},{"taxonomy":"tribe_events_cat","embeddable":true,"href":"https:\/\/bifi.es\/es\/wp-json\/wp\/v2\/tribe_events_cat?post=5159"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}